DNA

Part:BBa_K3971026

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-10-19)


Portion of the Neutral Site 3 of the Synechococcus elongatus UTEX 2973 genome.

This part is a portion of the Neutral Site 3 of the Synechococcus elongatus UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 3 using the pSL2680 plasmid, which is a Cpf1 based CRISPR plasmid [1]. This was one of the homology arms to Neutral Site 3 in our composite part BBa_K3971028 and the composite part BBa_K3971030 which were designed to insert sps and spp genes in the Neutral Site 3 of the S. elongatus genome respectively, under different promoters.


References

1: Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria. Ungerer J, Pakrasi HB. Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681. 10.1038/srep39681 PubMed 28000776

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 635
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 635
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 635
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 635
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None